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KMID : 0545120010110030534
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Journal of Microbiology and Biotechnology
2001 Volume.11 No. 3 p.534 ~ p.538
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Asparagine Residue at Position 71 is Responsible for Alkali-Tolerance of the Xylanase from Bacillus pumilus A-30
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LIU, XIANG-MEI
QI, MENG/LIN, JIAN-QIANG/WU, ZHI-HONG/QU, YIN-BO
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Abstract
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The xynA gene encoding an alkali-tolerant endo-1, 4-β-xylanase (XYN) was cloned from the alkalophilic Bacillus pumilus A-30. The nucleotide sequence of a 974-bp DNA fragment containing the xynA was determined. An ORF of 684 nucleotides that encoded a protein of 228 amino acids was detected. Asparagine-71 of XYN from B. pumilus A-30 showed to be highly conservative in alkaline xylanases of family G/11, upon comparing the amino acid sequences of 17 family G/11 xylanases. Site-directed mutation of N71D of the xynA gene resulted in a decrease of 12.4% in the specific activity and a significant decline in the enzyme activity in the alkaline pH range.
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